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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2012

Author(s): Käser, T; Müllebner, A; Hartl, RT; Essler, SE; Saalmüller, A; Catharina Duvigneau, J

Title: Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules.

Source: Cytokine. 2012; 60(2):400-409



Authors Vetmeduni Vienna:

Duvigneau Catharina
Hammer Sabina
Hartl Romana
Käser Tobias
Müllebner Andrea
Saalmüller Armin

Vetmed Research Units
Institute for Medical Biochemistry
Institute of Immunology


Abstract:
T-helper (TH) and regulatory T cells (Tregs) are important modulators of immune responses. Aim of this study was to analyse their expression potential for cytokines and other immune-relevant molecules. Therefore, porcine PBMC, CD4(-) cells, CD4(+)CD25(-) resting, CD4(+)CD25(dim) activated TH cells, and CD4(+)CD25(high) Tregs were analysed on their mRNA expression potential ex vivo or after in vitro stimulation with CD3 and IL-2 by RT-qPCR. In vitro stimulation led to an increased production of pro-inflammatory (IL-6, TNFα) and TH (IL-2, IL-4, IL-17, IFN-γ) cytokines and a diverse production of immunosuppressive cytokines (IL-10 and TGF-β) in PBMC, CD4(-), and CD4(+) cells. Resting and activated TH cells showed an increased expression of various immune-modulatory molecules indicating that porcine TH cells possess distinct immunological skills in order to react on the actual immune situation. In contrast, Tregs appear to fulfil mainly immunosuppressive functions characterized by increased production of IL-10, IL-35, CD40L, and CD25.

Keywords Pubmed: Animals
Antigens, CD/genetics*
Antigens, CD/metabolism
Cytokines/genetics*
Cytokines/metabolism
Flow Cytometry
Gene Expression Regulation*
RNA, Messenger/metabolism
Sus scrofa/genetics*
Sus scrofa/immunology*
T-Lymphocytes, Helper-Inducer/metabolism*
T-Lymphocytes, Regulatory/metabolism*


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