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Publication type: Journal Article
Document type: Full Paper

Year: 2002

Author(s): Armengol, E; Wiesmüller, KH; Wienhold, D; Büttner, M; Pfaff, E; Jung, G; Saalmüller, A

Title: Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus.

Source: J Gen Virol (83), Pt 3 551-560.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
To identify new T-cell epitopes of classical swine fever virus (CSFV), 573 overlapping, synthetic pentadecapeptides spanning 82% of the CSFV (strain Glentorf) genome sequence were synthesized and screened. In proliferation assays, 26 peptides distributed throughout the CSFV viral protein sequences were able to induce specific T-cell responses in PBMCs from a CSFV-Glentorf-infected d/d haplotype pig. Of these 26 peptides, 18 were also recognized by PBMCs from a CSFV-Alfort/187-infected d/d haplotype pig. In further experiments, it could be shown that peptide 290 (KHKVRNEVMVHWFDD), which corresponds to amino acid residues 1446-1460 of the CSFV non-structural protein NS2-3 could induce interferon-gamma secretion after secondary in vitro restimulation. The major histocompatibility complex (MHC) restriction for stimulation of T-cells by this pentadecapeptide was identified as being mainly MHC class II and partially MHC class I. In cytolytic assays, CSFV-specific cytotoxic T-lymphocytes (CTLs) were able to lyse peptide 290-loaded target cells. These findings indicate the existence of a CSFV-specific helper T-cell epitope and a CTL epitope in this peptide.

Keywords Pubmed: Amino Acid Sequence
Animals
Antibodies, Monoclonal/immunology
Antigens, Viral/chemistry*
Antigens, Viral/immunology*
Cell Division
Cells, Cultured
Classical swine fever virus/chemistry*
Classical swine fever virus/immunology*
Epitopes, T-Lymphocyte/chemistry
Epitopes, T-Lymphocyte/immunology*
Genome, Viral
Histocompatibility Antigens Class I/immunology
Histocompatibility Antigens Class II/immunology
Interferon-gamma/immunology
Interferon-gamma/secretion
Lymphocyte Activation
Mice
Molecular Sequence Data
Peptides/chemical synthesis
Peptides/chemistry
Peptides/immunology
Swine/immunology*
Swine/virology*
T-Lymphocytes, Cytotoxic/immunology
T-Lymphocytes, Cytotoxic/secretion
T-Lymphocytes, Helper-Inducer/immunology
T-Lymphocytes, Helper-Inducer/secretion
Viral Proteins/chemistry
Viral Proteins/immunology
Viral Structural Proteins/chemistry
Viral Structural Proteins/immunology


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