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Publication type: Journal Article
Document type: Full Paper

Year: 2001

Author(s): Freiberg, B; Höhlich, B; Haas, B; Saalmüller, A; Pfaff, E; Marquardt, O

Title: Type-independent detection of foot-and-mouth disease virus by monoclonal antibodies that bind to amino-terminal residues of capsid protein VP2.

Source: J Virol Methods (92), 2 199-205.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
The characterization of monoclonal antibodies raised against the foot-and-mouth disease virus isolates A22 Iraq/1964, Asia1 Shamir-Israel/1989, and SAT1 Zimbabwe/1989 with regard to neutralizing activity and sensitivity of their epitopes for treatment with trypsin, resulted in the identification of one non-neutralizing antibody in each panel that binds to a trypsin-sensitive epitope. Furthermore, each of these antibodies recognized 27 isolates of different provenance, representative of six serotypes. These antibodies are recommended for type-independent antigen detection by ELISA. The epitopes for these antibodies reside at the intertypically conserved N-terminus of capsid protein VP2. The two are specified by the lysines at positions two and three, but differ from each other as indicated by the variable heavy chain sequences of their antibodies.

Keywords Pubmed: Amino Acid Sequence
Antibodies, Monoclonal/metabolism*
Aphthovirus/classification
Aphthovirus/isolation & purification*
Aphthovirus/metabolism
Capsid/chemistry*
Capsid/genetics
Capsid/metabolism*
Capsid Proteins
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Epitopes
Foot-and-Mouth Disease/virology*
Neutralization Tests
Peptides/chemistry
Peptides/immunology
Trypsin/metabolism


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