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Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Brodersen, R; Bijlsma, F; Gori, K; Jensen, KT; Chen, W; Dominguez, J; Haverson, K; Moore, PF; Saalmüller, A; Sachs, D; Slierendrecht, WJ; Stokes, C; Vainio, O; Zuckermann, F; Aasted, B

Title: Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.

Source: Vet Immunol Immunopathol (64), 1 1-13.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions on the following markers: CD1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43; 44, 45, 45R, 46, 49, 61, 62L, TCR gamma/delta, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new leucocyte antigen reactive mAbs. (C) 1998 Elsevier Science B.V. Ah rights reserved.

Keywords Pubmed: Animals - : Antibodies, Monoclonal - immunology : Antibody Specificity - immunology : Antigens, Surface - chemistry : Cats - chemistry : Cattle - chemistry : Chickens - chemistry : Cross Reactions - chemistry : Dogs - chemistry : Guinea Pigs - chemistry : Histocompatibility Antigens Class II - immunology : Horses - immunology : Humans - immunology : Integrins - immunology : Leukocytes - immunology : Mink - immunology : Molecular Weight - immunology : Oncorhynchus mykiss - immunology : Precipitin Tests - immunology : Rabbits - immunology : Rats - immunology : Species Specificity - immunology : Swine - immunology

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