University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Summerfield, A; Saalmüller, A

Title: Interleukin-2 dependent selective activation of porcine gamma delta T lymphocytes by an extract from the leaves of Acanthospermum hispidum.

Source: Int J Immunopharmacol (20), 1-3 85-98.



Authors Vetmeduni Vienna:

Saalmüller Armin


Project(s): Immunstimulatorische Wirkung eines Pflanzenextraktes


Abstract:
The immunostimulatory capacity of Acanthospermum hispidum, a tropical plant which is used in the traditional medicine of Benin for the treatment of infectious diseases, was studied in the porcine immune system. These in vitro studies revealed the capacity of A. hispidum to enhance the proliferation of T lymphocytes after stimulation with ConA or allogeneic stimulator cells in the mixed leucocyte culture (MLC). The virus-specific MHC class II-restricted in vitro immune response against pseudorabies virus (PRV) was also enhanced in a co-stimulating manner. Phenotyping of T lymphocytes that had been activated in vitro in the presence of A. hispidum revealed an increase of activated gamma delta T lymphocytes with the phenotype CD2(-)CD5(low+)CD8(-). In vitro analysis of the influence on the lymphocyte function demonstrated neither an increase of the immunoglobulin synthesis, nor of the interleukin-2 production, nor of the cytolytic activities. Experiments using separated T-lymphocyte subpopulations showed that the co-stimulatory activity was based on a synergism between T helper and gamma delta T lymphocytes, and that gamma delta T lymphocytes were the targets of the plant-derived extract. The gamma delta T cells which could not be activated in mixed leukocyte cultures or with pseudorabies virus antigen in a secondary immune response, were reactive to the interleukin-2 released from antigen-stimulated T helper lymphocytes. A. hispidum enhanced the responsiveness of gamma delta T lymphocytes to IL-2. (C) 1998 International Society for Immunopharmacology. Published by Elsevier Science Ltd. All rights reserved.

Keywords Pubmed: Animals - : B-Lymphocytes - drug effects : Cells, Cultured - drug effects : Concanavalin A - pharmacology : Cytotoxicity, Immunologic - pharmacology : Epitopes - immunology : Immunoglobulins - biosynthesis : Interleukin-2 - biosynthesis : Interleukin-4 - biosynthesis : Lymphocyte Activation - drug effects : Lymphocyte Subsets - drug effects : Mice - drug effects : Mitogens - pharmacology : Phenotype - pharmacology : Plant Extracts - pharmacology : Plant Leaves - chemistry : Plant Lectins - chemistry : Plants, Medicinal - chemistry : Receptors, Antigen, T-Cell, gamma-delta - immunology : Stimulation, Chemical - immunology : Swine - immunology : T-Lymphocytes - drug effects : T-Lymphocytes, Helper-Inducer - drug effects

© University of Veterinary Medicine ViennaHelp and Downloads