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Publication type: Journal Article
Document type: Full Paper

Year: 1997

Author(s): Köhler, H; Lemser, B; Müller, G; Saalmüller, A

Title: Early changes in the phenotypic composition of lymphocytes in the bronchoalveolar lavage of pigs after aerogenic immunization with Pasteurella multocida aerosols.

Source: Vet Immunol Immunopathol (58), 3-4 277-286.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
The phenotypic composition of bronchoalveolar lymphocytes (BALL) of healthy untreated pigs and of pigs immunized via aerosol with a live Pasteurella multocida mutant was measured cytofluorometrically in comparison to blood lymphocytes (PBL). Cells were analyzed using monoclonal antibodies against porcine IgM, IgA, Ige and SWC3 in single-colour fluorescence and the following antibody combinations for two-colour fluorescence: CD2/SWC1, SWC1/CD25 and CD4/CD8. In nonimmunized pigs, differences between lymphocyte subsets derived from blood and lung were obvious. The numbers of CD2(-)SWC1(+) T-cells, CD25(+) activated lymphocytes and IgG(+) cells were significantly higher in the bronchoalveolar compartment. Aerosol immunization with P. multocida caused only insignificant changes in the phenotypic composition of PBL whereas the composition of lung-derived lymphocytes changed markedly. The percentage of CD2(-)SWC1(+) lymphocytes and of CD25(+) cells coexpressing the SWC1 antigen in BALL increased up to 10 days after aerosol administration, whereas the percentage of all Ig(+) cells decreased. These data reflect an early cellular reaction to an aerogenic immunization with a P. multocida aerosol, characterized by an increase in CD2(-) T-cells and a T-cell activation. (C) 1997 Elsevier Science B.V.

Keywords Pubmed: Aerosols - : Animals - : Bronchoalveolar Lavage Fluid - immunology : Immunization - immunology : Immunophenotyping - immunology : Lymphocytes - immunology : Pasteurella multocida - immunology : Swine - immunology

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