University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1996

Author(s): Summerfield, A; Rziha, HJ; Saalmüller, A

Title: Functional characterization of porcine CD4+CD8+ extrathymic T lymphocytes.

Source: Cell Immunol (168), 2 291-296.

Authors Vetmeduni Vienna:

Saalmüller Armin

The porcine immune system is unique in that the expression of CD4 and CD8 antigens defines four subpopulations of resting, extrathymic (CD1(-)) T lymphocytes, In addition to CD4(-)CD8(+) and CD4(+)CD8(-) T lymphocytes, CD4(-)CD8(-) and CD4(+)CD8(+) lymphocyte subpopulations are prominent in blood as well as in lymphoid tissues, In the present study, a functional comparison was made between CD4(+)CD8(-) and CD4(+)CD8(+) T lymphocyte subpopulations. In a primary in vitro immune response against alloantigenic stimulator cells, both subpopulations proliferated without significant differences in their reactivity, Different results were obtained when analyzing the antigen-specific functions of the two CD4(+) subpopulations in a secondary response against recall viral antigen; these experiments were performed with T lymphocytes from pseudorabies virus-immunized pigs, The proliferative response against viral antigens could be assigned to the CD4(+)CD8(+) subpopulation, whereas the CD4(+)CD8(-) subpopulation remained nonreactive. Further analyses of the virus-specific in vitro immune response revealed a major histocompatibility complex (MHC) class II restricted helper T lymphocyte reaction involving CD4 but not CDS molecules as restriction elements, Taken together, these results demonstrate that only the extrathymic CD4(+)CD8(+) T lymphocyte subpopulation of swine contains MHC class II-restricted antigen-specific memory T helper cells. (C) 1996 Academic Press, Inc.

Keywords Pubmed: Animals - : Antigens, CD4 - : Antigens, CD8 - : Antigens, Viral - immunology : Cell Division - immunology : Cells, Cultured - immunology : Flow Cytometry - immunology : Histocompatibility Antigens Class II - immunology : Immunization - immunology : Immunologic Memory - immunology : Immunophenotyping - immunology : Lymphocyte Activation - immunology : Pseudorabies - immunology : Swine - immunology : Swine, Miniature - blood : T-Lymphocyte Subsets - classification : T-Lymphocytes, Helper-Inducer - immunology

© University of Veterinary Medicine ViennaHelp and Downloads