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Publication type: Journal Article
Document type: Full Paper

Year: 1995

Author(s): Pauly, T; Elbers, K; König, M; Lengsfeld, T; Saalmüller, A; Thiel, HJ

Title: Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope.

Source: J Gen Virol (76 ( Pt 12)), 1 3039-3049.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
Classical swine fever virus (CSFV)-specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4(-)CD6(+)CD8(+) MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.

Keywords Pubmed: Amino Acid Sequence - : Animals - : Antigens, CD - analysis : Antigens, CD4 - analysis : Antigens, CD8 - physiology : Antigens, Differentiation, T-Lymphocyte - analysis : Cell Line, Transformed - analysis : Classical Swine Fever - immunology : Classical swine fever virus - immunology : Cytotoxicity Tests, Immunologic - immunology : Epitope Mapping - immunology : Epitopes, T-Lymphocyte - analysis : Molecular Sequence Data - analysis : Peptides - chemical synthesis : Swine - chemical synthesis : Swine, Miniature - chemical synthesis : T-Lymphocyte Subsets - chemistry : T-Lymphocytes, Cytotoxic - immunology : Vaccinia virus - genetics : Viral Nonstructural Proteins - genetics

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