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Type of publication: Diploma Thesis
Type of document:

Year: 2012

Authors: Wlaschitz, Sabrina

Title: Phytochemische Charakterisierung phenolischer Inhaltsstoffe verschiedener Plantago-Arten.

Other title: Phytochemical characterisation of phenolic compounds of different Plantago species

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 54.


Chizzola Remigius

Rausch Wolf-Dieter

Vetmed Research Units:
Institute of Animal Nutrition and Functional Plant Compounds

Graduation date: 24.10.12

Project(s): PLANT food supplements: Levels of Intake, Benefit and Risk Assessment

The positive properties of genus Plantago are used in many different ways. It is used as a phytotherapeutic to treat respiratory diseases in human and animals, it is used in animal feeding and it is part of dietary supplements. For this thesis the phenolic compounds of twelve different Plantago species were analyzed by HPLC and the total content of phenolics and the antioxidant activity were measured by spectrophotometric methods. Differences and similarities of both, the various species and their different parts (leaves, stem with flowers/fruits, roots) were shown. The HPLC analyses showed that a wide range of phenolic compounds was present. The phenylethanoid glycoside verbascoside was identified as a marker for genus Plantago, because it was found in all samples, but it couldnÂ’t be used to differentiate between the different species. In most samples verbascoside was the main phenolic compound, but there were some cases where other compounds (peak H and J) with similar UV-spectrum dominated. It was shown, that the content of different phenolic substances, the total content of phenolics and the antioxidant activity varies between and within the individual Plantago species and between the analyzed parts of the plants. This makes it impossible to give a definite statement about the correlation between phenolic compounds and effects. Therefore tests with a larger amount of plant material and more information would be needed. However, the roots should get more attention by using Plantago because partially they showed a higher antioxidant potency than aerial parts of the plant.

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