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Type of publication: Journal Article
Type of document: Full Paper

Year: 2012

Authors: Gad, A; Hoelker, M; Besenfelder, U; Havlicek, V; Cinar, U; Rings, F; Held, E; Dufort, I; Sirard, MA; Schellander, K; Tesfaye, D

Title: Molecular mechanisms and pathways involved in bovine embryonic genome activation and their regulation by alternative in vivo and in vitro culture conditions.

Source: Biol Reprod. 2012; 87(4):100



Authors Vetmeduni Vienna:

Besenfelder Urban
Havlicek Vitezslav

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology


Abstract:
Understanding gene expression patterns in response to altered environmental conditions at different time points of the preimplantation period would improve our knowledge on regulation of embryonic development. Here we aimed to examine the effect of alternative in vivo and in vitro culture conditions at the time of major embryonic genome activation (EGA) on the development and transcriptome profile of bovine blastocysts. Four different blastocyst groups were produced under alternative in vivo and in vitro culture conditions before or after major EGA. Completely in vitro- and in vivo-produced blastocysts were used as controls. We compared gene expression patterns between each blastocyst group and in vivo blastocyst control group using EmbryoGENE"s bovine microarray. The data showed that changing culture conditions from in vivo to in vitro or vice versa, either before or after the time of major EGA, had no effect on the developmental rates; however, in vitro conditions during that time critically influenced the transcriptome of the blastocysts produced. The source of oocyte had a critical effect on developmental rates and the ability of the embryo to react to changing culture conditions. Ontological classification highlighted a marked contrast in expression patterns for lipid metabolism and oxidative stress response between blastocysts generated in vivo versus in vitro, with opposite trends. Molecular mechanisms and pathways that are influenced by altered culture conditions during EGA were defined. These results will help in the development of new strategies to modify culture conditions at this critical stage to enhance the development of competent blastocysts.

Keywords Pubmed: Animals
Cattle/embryology*
Cattle/genetics*
Cattle/metabolism
Cells, Cultured
Culture Media/pharmacology
Embryo Culture Techniques
Embryo, Mammalian/cytology
Embryo, Mammalian/drug effects
Embryo, Mammalian/metabolism*
Embryonic Development/drug effects
Embryonic Development/genetics
Embryonic Development/physiology
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental*/drug effects
Gene Regulatory Networks
Genome*/drug effects
Genome*/physiology
Microarray Analysis
Pregnancy
Signal Transduction/genetics
Signal Transduction/physiology
Validation Studies as Topic


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