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Publication type: Journal Article
Document type: Full Paper

Year: 1991

Author(s): Bühring, HJ; Saalmüller, A; Müller, C; van Agthoven, AJ; Busch, FW

Title: The monoclonal antibody 11G7 recognizes a novel differentiation antigen expressed on hemopoietic precursor cells.

Source: Hybridoma (10), 1 77-88.



Authors Vetmeduni Vienna:

Saalmüller Armin


Abstract:
A monoclonal antibody (11G7) detecting a novel antigen on human hemopoietic progenitor cells (named 11G7R = 11G7 receptor) was raised by immunization of a Balb/c mouse with the leukemic blasts of a patient suffering from chronic myelogenous leukemia blast crisis (CML-BC). The antigen is expressed on most of MHC class II bearing peripheral blood leucocytes (PBL) and on a subpopulation of bone marrow mononuclear cells (BMMNC). By FACS-sorting and colony assays, it could be demonstrated that 11G7R is expressed on myelo-monocytic and myelo-granulocytic bone marrow precursor cells (GM-CFC, G-CFC, M-CFC) but is absent from erythroid precursor cells (BFU-E) and on cells exhibiting the capacity to form mixed colonies (GEMM-CFC). Double-fluorescence analysis on BMMNC revealed that 11G7R is expressed on a subset of B-cells, myeloid cells and cells carrying the HPCA-1 antigen (CD34). It has a similar distribution pattern to the myeloid antigens CD13 and CD33. However, in contrast to these antigens, 11G7R is also expressed on the blasts of several lymphoid leukemias (4/9 B-ALL, 1/2 T-ALL) and therefore it is not restricted to the myeloid lineage.

Keywords Pubmed: Animals - : Antibodies, Monoclonal - : Antigens, Differentiation - chemistry : Hematopoietic Stem Cells - immunology : Humans - immunology : Hybridomas - immunology : Immunochemistry - immunology : Immunoglobulin G - immunology : Leukemia - immunology : Leukocytes, Mononuclear - immunology : Mice - immunology : Tumor Cells, Cultured - immunology

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