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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Mair, KH; Müllebner, A; Essler, SE; Duvigneau, JC; Storset, AK; Saalmüller, A; Gerner, W

Title: Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.

Source: Vet Res. 2013; 44:13



Authors Vetmeduni Vienna:

Duvigneau Catharina
Gerner Wilhelm
Hammer Sabina
Mair Kerstin
Müllebner Andrea
Saalmüller Armin

Vetmed Research Units
Institute for Medical Biochemistry
Institute of Immunology


Abstract:
Natural Killer (NK) cells play a crucial role in the early phase of immune responses against various pathogens. In swine so far only little information about this lymphocyte population exists. Phenotypical analyses with newly developed monoclonal antibodies (mAbs) against porcine NKp46 recently revealed that in blood NKp46- and NKp46+ cells with NK phenotype exist with comparable cytotoxic properties. In spleen a third NKp46-defined population with NK phenotype was observed that was characterised by a low to negative CD8α and increased NKp46 expression. In the current study it is shown that this NKp46high phenotype was correlated with an increased expression of CD16 and CD27 compared to the CD8α+NKp46- and NKp46+ NK-cell subsets in spleen and blood. Additionally NKp46high NK cells expressed elevated levels of the chemokine receptor CXCR3 on mRNA level. Functional analyses revealed that splenic NKp46high NK cells produced much higher levels of Interferon-γ and Tumor Necrosis Factor-α upon stimulation with cytokines or phorbol-12-myristate-13-acetate/Ionomycin compared to the other two subsets. Furthermore, cross-linking of NKp46 by NKp46-specific mAbs led to a superior CD107a expression in the NKp46high NK cells, thus indicating a higher cytolytic capacity of this subset. Therefore porcine splenic NKp46high NK cells represent a highly activated subset of NK cells and may play a profound role in the immune surveillance of this organ.

Keywords Pubmed: Animals
Antigens, CD8/metabolism
Cytokines/biosynthesis
Cytokines/immunology
Gene Expression Regulation*
Interferon-gamma/genetics
Interferon-gamma/metabolism
Ionomycin/pharmacology
Lymphocytes/immunology*
Natural Cytotoxicity Triggering Receptor 1/immunology*
Phorbol Esters/pharmacology
RNA, Messenger/genetics
RNA, Messenger/metabolism
Spleen/immunology*
Sus scrofa/immunology*
Tumor Necrosis Factor-alpha/biosynthesis
Tumor Necrosis Factor-alpha/immunology


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