University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Open Access Logo

Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Reutner, K; Leitner, J; Müllebner, A; Ladinig, A; Essler, SE; Duvigneau, JC; Ritzmann, M; Steinberger, P; Saalmüller, A; Gerner, W

Title: CD27 expression discriminates porcine T helper cells with functionally distinct properties.

Source: Vet Res. 2013; 44:18



Authors Vetmeduni Vienna:

Duvigneau Catharina
Gerner Wilhelm
Hammer Sabina
Ladinig Andrea
Müllebner Andrea
Reutner Katharina
Ritzmann Mathias
Saalmüller Armin

Vetmed Research Units
Institute for Medical Biochemistry
Institute of Immunology
University Clinic for Swine


Abstract:
Differentiation of porcine T helper cells is still poorly investigated, partly due to a lack of monoclonal antibodies (mAbs) specific for molecules involved in this process. Recently, we identified a mAb specific for porcine CD27 and showed that CD27 is expressed by all naïve CD8α- T helper cells but divides CD8α+ T helper cells into a CD27+ and a CD27- subset. In the present study, detailed phenotypical and functional analyses of these T-helper cell subpopulations were performed. Naïve CD8α-CD27+ T helper cells predominantly resided in various lymph nodes, whereas higher proportions of CD8α+CD27+ and CD8α+CD27- T helper cells were found in blood, spleen and liver. Both CD8α+CD27+ and CD8α+CD27- T helper cells were capable of producing IFN-γ upon in vitro polyclonal stimulation and antigen-specific restimulation. Experiments with sorted CD8α-CD27+, CD8α+CD27+ and CD8α+CD27- T-helper cell subsets following polyclonal stimulation revealed the lowest proliferative response but the highest ability for IFN-γ and TNF-α production in the CD8α+CD27- subset. Therefore, these cells resembled terminally differentiated effector memory cells as described in human. This was supported by analyses of CCR7 and CD62L expression. CD8α+CD27- T helper cells were mostly CCR7- and had considerably reduced CD62L mRNA levels. In contrast, expression of both homing-receptors was increased on CD8α+CD27+ T helper cells, which also had a proliferation rate similar to naïve CD8α-CD27+ T helper cells and showed intermediate levels of cytokine production. Therefore, similar to human, CD8α+CD27+ T helper cells displayed a phenotype and functional properties of central memory cells.

Keywords Pubmed: Animals
Antigens, CD27/chemistry
Antigens, CD27/genetics
Antigens, CD27/immunology*
Cell Differentiation
Lymphocyte Activation*
Molecular Sequence Data
Sequence Alignment/veterinary
Sequence Analysis, Protein/veterinary
Sus scrofa/immunology*
T-Lymphocyte Subsets/cytology
T-Lymphocyte Subsets/immunology
T-Lymphocyte Subsets/metabolism
T-Lymphocytes/cytology*
T-Lymphocytes/immunology
T-Lymphocytes/metabolism


© University of Veterinary Medicine ViennaHelp and Downloads