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Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Worliczek, HL; Ruttkowski, B; Schwarz, L; Witter, K; Tschulenk, W; Joachim, A

Title: Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.

Source: PLoS One. 2013; 8(7):e69797

Authors Vetmeduni Vienna:

Joachim Anja
Ruttkowski Bärbel
Schwarz Lukas
Witter Kirsti
Worliczek Hanna

Vetmed Research Units
Institute of Morphology
Institute of Parasitology
University Clinic for Swine

Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is Toxoplasma gondii. Although this parasite is well investigated there is no adequate in vitro model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. The use of Isosporasuis, a sister taxon to T. gondii and the causative agent of piglet coccidiosis, could provide a solution for this. In the present study an in vitro model for neonatal porcine coccidiosis in cells representative for the in vivo situation in the piglet gut was developed and evaluated. The parasite development was investigated by light and transmission electron microscopy and optimum culture conditions were evaluated. Intestinal porcine epithelial cells (IPEC-J2) adequately representing the natural host cells supported the development of all endogenous life cycle stages of I. suis, including gametocytes and oocysts. A concentration of 5% fetal calf serum in the culture medium led to highest gametocyte densities on day 12 post infection. Low infection doses (≤1 sporozoite for 100 host cells) were best for oocyst and gametocyte development. The presented system can also be used for immunostaining with established antibodies developed against T. gondii (in our case, anti-TgIMC3 antibodies directed against the inner membrane complex 3). The complete life cycle of I. suis in a cell line representing the natural host cell type and species provides a unique model among coccidian parasites and can be used to address a wide range of topics, especially with regard to the sexual development of coccidia.

Keywords Pubmed: Animals
Cell Culture Techniques*
Epithelial Cells/parasitology*
Intestinal Mucosa/cytology
Intestinal Mucosa/parasitology
Isospora/growth & development*
Life Cycle Stages
Time Factors

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