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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Flickinger, I; Rütgen, BC; Gerner, W; Calice, I; Tichy, A; Saalmüller, A; Kleiter, M

Title: Radiation up-regulates the expression of VEGF in a canine oral melanoma cell line.

Source: J Vet Sci. 2013; 14(2):207-214

Authors Vetmeduni Vienna:

Flickinger Irene
Gerner Wilhelm
Kleiter Miriam
Rütgen Barbara
Saalmüller Armin
Tichy Alexander

Vetmed Research Units
Clinical Pathology Platform
University Clinic for Small Animals, Clinical Unit of Internal Medicine Small Animals
Institute of Immunology
Platform Bioinformatics and Biostatistics

To evaluate radiosensitivity and the effects of radiation on the expression of vascular endothelial growth factor (VEGF) and VEGF receptors in the canine oral melanoma cell line, TLM 1, cells were irradiated with doses of 0, 2, 4, 6, 8 and 10 Gray (Gy). Survival rates were then determined by a MTT assay, while vascular endothelial growth factor receptor (VEGFR)-1 and -2 expression was measured by flow cytometry and apoptotic cell death rates were investigated using an Annexin assay. Additionally, a commercially available canine VEGF ELISA kit was used to measure VEGF. Radiosensitivity was detected in TLM 1 cells, and mitotic and apoptotic cell death was found to occur in a radiation dose dependent manner. VEGF was secreted constitutively and significant up-regulation was observed in the 8 and 10 Gy irradiated cells. In addition, a minor portion of TLM 1 cells expressed vascular endothelial growth factor receptor (VEGFR)-1 intracellularly. VEGFR-2 was detected in the cytoplasm and was down-regulated following radiation with increasing dosages. In TLM 1 cells, apoptosis plays an important role in radiation induced cell death. It has also been suggested that the significantly higher VEGF production in the 8 and 10 Gy group could lead to tumour resistance.

Keywords Pubmed: Animals
Apoptosis/radiation effects*
Cell Line, Tumor/radiation effects
Dose-Response Relationship, Radiation
Enzyme-Linked Immunosorbent Assay/veterinary
Mouth Neoplasms/genetics
Mouth Neoplasms/metabolism
Radiation Tolerance
Tetrazolium Salts/metabolism
Up-Regulation/radiation effects*
Vascular Endothelial Growth Factor A/genetics
Vascular Endothelial Growth Factor A/metabolism
Vascular Endothelial Growth Factor A/radiation effects*
Vascular Endothelial Growth Factor Receptor-1/genetics
Vascular Endothelial Growth Factor Receptor-1/metabolism
Vascular Endothelial Growth Factor Receptor-1/radiation effects*
Vascular Endothelial Growth Factor Receptor-2/genetics
Vascular Endothelial Growth Factor Receptor-2/metabolism
Vascular Endothelial Growth Factor Receptor-2/radiation effects*

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