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Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Lima, AS; Schimmel, J; Lukas, B; Novak, J; Barroso, JG; Figueiredo, AC; Pedro, LG; Degenhardt, J; Trindade, H

Title: Genomic characterization, molecular cloning and expression analysis of two terpene synthases from Thymus caespititius (Lamiaceae).

Source: Planta. 2013; 238(1):191-204

Authors Vetmeduni Vienna:

Lukas Brigitte
Novak Johannes

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds

The identification, isolation and functional characterization of two genes encoding two monoterpene synthases-γ-terpinene synthase (Tctps2) and α-terpineol synthase (Tctps5)-from three chemically distinct Thymus caespititius (Lamiaceae) genotypes were performed. Genomic exon-intron structure was also determined for both terpene synthase genes, revealing an organization with seven exons and six introns. The cDNA of Tctps2 was 2,308 bp long and had an open reading frame of 1,794 bp encoding for a protein with 598 amino acids. Tctps5 was longer, mainly due to intron sequences, and presented high intraspecific variability on the plants analyzed. It encoded for a protein of 602 amino acids from an open reading frame of 1,806 bp comprising a total of 2,507 bp genomic sequence. The amino acid sequence of these two active Tctps genes shared 74 % pairwise identity, ranging between 42 and 94 % similarity with about 50 known terpene synthases of other Lamiaceae species. Gene expression revealed a multi-product Tctps2 and Tctps5 enzymes, producing γ-terpinene and α-terpineol as major components, respectively. These enzymatic results were consistent with the monoterpene profile present in T. caespititius field plants, suggesting a transcriptional regulation in leaves. Herewith reported for the first time for this species, these two newly characterized Tctps genes improve the understanding of the molecular mechanisms of reaction responsible for terpene biosynthesis and chemical diversity found in T. caespititius.

Keywords Pubmed: Alkyl and Aryl Transferases/genetics*
Alkyl and Aryl Transferases/metabolism
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Gene Expression Regulation, Plant
Molecular Sequence Data
Plant Proteins/genetics
Plant Proteins/metabolism
Sequence Homology, Amino Acid

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