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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Mann, E; Hein, I; Mester, P; Stessl, B; Rossmanith, P; Wagner, M; Dzieciol, M

Title: A Robust and Poisson Validated Quantitative 5' Nuclease TaqManA (R) Real-Time PCR Assay Targeting fimA for the Rapid Detection of Salmonella spp. in Food.

Source: Food Anal Method (6), 4 991-995.

Authors Vetmeduni Vienna:

Dzieciol Monika
Hein Ingeborg
Mester Patrick-Julian
Rossmanith Peter
Selberherr Evelyne
Stessl Beatrix
Wagner Martin

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology

Project(s): Preventive Veterinary Medicine

A newly designed TaqManA (R) probe and an internal amplification control were implemented in a conventional PCR system targeting the major fimbrial subunit encoding gene fimA. This assay has an inclusivity and exclusivity of 100 % (n = 126). The limit of detection (LOD) and the absolute quantification limit, both determined by advanced Poisson analyses, were three bacterial cell equivalents per quantitative real-time PCR reaction. The fimA assay achieved 100 % accuracy and performed very robustly, producing reproducible, reliable data for quantification of Salmonella spp., even at the LOD.

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