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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Balka, G; Ladinig, A; Ritzmann, M; Saalmüller, A; Gerner, W; Käser, T; Jakab, C; Rusvai, M; Weißenböck, H

Title: Immunohistochemical characterization of type II pneumocyte proliferation after challenge with type I porcine reproductive and respiratory syndrome virus.

Source: J Comp Pathol. 2013; 149(2-3):322-330

Authors Vetmeduni Vienna:

Gerner Wilhelm
Käser Tobias
Ladinig Andrea
Ritzmann Mathias
Saalmüller Armin
Weissenböck Herbert

Vetmed Research Units
Institute of Immunology
Institute of Pathology
University Clinic for Swine

The aim of this study was to characterize histologically and immunohistochemically the lung lesions developing in growing pigs, 10 and 21 days after experimental challenge with a field strain of porcine reproductive and respiratory syndrome virus (PRRSV). Lung lesions were scored for (1) pneumocyte hypertrophy and hyperplasia, (2) septal mononuclear infiltration, (3) intra-alveolar necrotic debris, (4) intra-alveolar inflammatory cell accumulation and (5) perivascular inflammatory cell accumulation. Immunohistochemistry was performed using antibodies specific for cytokeratin, Ki67, thyroid transcription factor (TTF)-1, the myelomonocytic marker MAC387 and PRRSV. Anti-TTF-1 identified type II pneumocytes and there was marked proliferation of these cells compared with control lung (P <0.05). Anti-cytokeratin labelled type I and II pneumocytes as well as bronchial epithelial cells; however, this labelling was not suitable for cell counting purposes. There was a correlation between lesion severity and the number of cells expressing Ki67 (P <0.05).

Keywords Pubmed: Animals
Enzyme-Linked Immunosorbent Assay
Porcine Reproductive and Respiratory Syndrome/metabolism*
Porcine Reproductive and Respiratory Syndrome/pathology
Porcine respiratory and reproductive syndrome virus
Reverse Transcriptase Polymerase Chain Reaction

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