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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Balka, G; Ladinig, A; Ritzmann, M; Saalmüller, A; Gerner, W; Käser, T; Jakab, C; Rusvai, M; Weißenböck, H

Title: Immunohistochemical characterization of type II pneumocyte proliferation after challenge with type I porcine reproductive and respiratory syndrome virus.

Source: J Comp Pathol. 2013; 149(2-3):322-330



Authors Vetmeduni Vienna:

Gerner Wilhelm
Käser Tobias
Ladinig Andrea
Ritzmann Mathias
Saalmüller Armin
Weissenböck Herbert

Vetmed Research Units
Institute of Immunology
Institute of Pathology
University Clinic for Swine


Abstract:
The aim of this study was to characterize histologically and immunohistochemically the lung lesions developing in growing pigs, 10 and 21 days after experimental challenge with a field strain of porcine reproductive and respiratory syndrome virus (PRRSV). Lung lesions were scored for (1) pneumocyte hypertrophy and hyperplasia, (2) septal mononuclear infiltration, (3) intra-alveolar necrotic debris, (4) intra-alveolar inflammatory cell accumulation and (5) perivascular inflammatory cell accumulation. Immunohistochemistry was performed using antibodies specific for cytokeratin, Ki67, thyroid transcription factor (TTF)-1, the myelomonocytic marker MAC387 and PRRSV. Anti-TTF-1 identified type II pneumocytes and there was marked proliferation of these cells compared with control lung (P <0.05). Anti-cytokeratin labelled type I and II pneumocytes as well as bronchial epithelial cells; however, this labelling was not suitable for cell counting purposes. There was a correlation between lesion severity and the number of cells expressing Ki67 (P <0.05).

Keywords Pubmed: Animals
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Pneumocytes/metabolism*
Pneumocytes/pathology
Porcine Reproductive and Respiratory Syndrome/metabolism*
Porcine Reproductive and Respiratory Syndrome/pathology
Porcine respiratory and reproductive syndrome virus
Reverse Transcriptase Polymerase Chain Reaction
Swine


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