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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1996

Author(s): Hussein, AA; Schmoll, F; F├╝hrer, F; Brem, G; Schellander, K

Title: Evaluation of seven microsatellite loci in Simmental cattle.

Source: Zentralbl Veterinarmed A (43), 1 1-8.



Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Seven microsatellite loci were evaluated for their suitability for parentage control. The polymerase chain reaction (PCR) was used to amplify the short tandem repeat (STR) loci in separate reactions. The microsatellite polymorphisms were visualized by radioisotopic autoradiographic detection. The microsatellite loci showed extensive polymorphism with allele numbers ranging from 4-23 and polymorphism information content (PIC) values in the range of 0.57-0.87. The analysis of these loci also revealed that they have a 99.9% combined probability of exclusion (PE) of erroneous parentage. The results of this study revealed that a very high probability of exclusion could be reached with only four microsatellite loci.

Keywords Pubmed: Alleles
Animals
Base Sequence
Breeding
Cattle/genetics*
DNA/analysis
DNA/chemistry
DNA/genetics*
DNA Primers/chemistry
Female
Gene Frequency
Male
Microsatellite Repeats/genetics*
Molecular Sequence Data
Polymorphism, Genetic


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