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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 1995

Authors: Hilbert, F; Mayr, B; Lackner, F; Bauer, F

Title: The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis.

Source: Electrophoresis (16), 6 903-904.



Authors Vetmeduni Vienna:

Bauer Friedrich
Hilbert Friederike

Vetmed Research Units
Institute of Animal Breeding and Genetics
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Hygiene and Technology


Abstract:
The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12-20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, Taq-DNA polymerase) interfere with silver staining of nucleic acids.

Keywords Pubmed: DNA/analysis*
Electrophoresis, Polyacrylamide Gel*
Muscles/cytology
Polymerase Chain Reaction/methods*
Proteins/chemistry*
Silver Staining*


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