University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 1995

Authors: Hilbert, F; Mayr, B; Lackner, F; Bauer, F

Title: The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis.

Source: Electrophoresis (16), 6 903-904.

Authors Vetmeduni Vienna:

Bauer Friedrich
Hilbert Friederike

Vetmed Research Units
Institute of Animal Breeding and Genetics
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Hygiene and Technology

The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12-20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, Taq-DNA polymerase) interfere with silver staining of nucleic acids.

Keywords Pubmed: DNA/analysis*
Electrophoresis, Polyacrylamide Gel*
Polymerase Chain Reaction/methods*
Silver Staining*

© University of Veterinary Medicine ViennaHelp and DownloadsAccessibility statement