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Publication type: Journal Article
Document type: Full Paper

Year: 2013

Author(s): Hollmann, M; Miller, I; Hummel, K; Sabitzer, S; Metzler-Zebeli, BU; Razzazi-Fazeli, E; Zebeli, Q

Title: Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.

Source: PLoS One. 2013; 8(12):e81602



Authors Vetmeduni Vienna:

Hollmann Manfred
Hummel Karin
Metzler-Zebeli Barbara
Miller Ingrid
Razzazi-Fazeli Ebrahim
Weigelsperger Sonja
Zebeli Qendrim

Vetmed Research Units
Institute for Medical Biochemistry
Institute of Animal Nutrition and Functional Plant Compounds
University Clinic for Swine
VetCore


Abstract:
Energy-rich diets can challenge metabolic and protective functions of the rumen epithelial cells, but the underlying factors are unclear. This study sought to evaluate proteomic changes of the rumen epithelium in goats fed a low, medium, or high energy diet. Expression of protein changes were compared by two-dimensional differential gel electrophoresis followed by protein identification with matrix assisted laser desorption ionisation tandem time-of-flight mass spectrometry. Of about 2,000 spots commonly detected in all gels, 64 spots were significantly regulated, which were traced back to 24 unique proteins. Interestingly, the expression profiles of several chaperone proteins with important cellular protective functions such as heat shock cognate 71 kDa protein, peroxiredoxin-6, serpin H1, protein disulfide-isomerase, and selenium-binding protein were collectively downregulated in response to high dietary energy supply. Similar regulation patterns were obtained for some other proteins involved in transport or metabolic functions. In contrast, metabolic enzymes like retinal dehydrogenase 1 and ATP synthase subunit beta, mitochondrial precursor were upregulated in response to high energy diet. Lower expressions of chaperone proteins in the rumen epithelial cells in response to high energy supply may suggest that these cells were less protected against the potentially harmful rumen toxic compounds, which might have consequences for rumen and systemic health. Our findings also suggest that energy-rich diets and the resulting acidotic insult may render rumen epithelial cells more vulnerable to cellular damage by attenuating their cell defense system, hence facilitating the impairment of rumen barrier function, typically observed in energy-rich fed ruminants.

Keywords Pubmed: Animal Feed/analysis
Animals
Diet*
Electrophoresis, Gel, Two-Dimensional
Energy Intake/genetics*
Energy Intake/immunology
Epithelium/immunology
Epithelium/metabolism*
Gene Expression Profiling
Gene Expression Regulation
Goats/immunology
Goats/metabolism*
Molecular Chaperones/genetics
Molecular Chaperones/immunology
Proteome/genetics*
Proteome/immunology
Rumen/immunology
Rumen/metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


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