The aim of this study was to evaluate the pressure distribution under three different types of harnesses used for guide dogs (designated H1, H2 and H3). The dogs (n = 8) led a trainer through a course including a range of exercises (straight line, curve left, curve right, upstairs and downstairs). All dogs were clinically sound and showed no sign of lameness. The pressures beneath the harnesses were determined by sensor strips and related to the gait. In all harnesses, the highest pressures were found in the right sternal region (H1 2.02 ± 0.6N/cm(2); H2 1.76 ± 0.4N/cm(2); H3 1.14 ± 0.5 N/cm(2)). In all other regions, the pressures were in the range of 0-1.32 N/cm(2). The right and left sternal regions were almost constantly loaded. Contrary to previous assumptions, the back regions had minimal loading. This investigation demonstrated that there were significant differences among the harnesses.