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Publication type: Journal Article
Document type: Full Paper

Year: 2014

Author(s): Mann, E; Schmitz-Esser, S; Zebeli, Q; Wagner, M; Ritzmann, M; Metzler-Zebeli, BU

Title: Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus.

Source: PLoS One. 2014; 9(1):e86950



Authors Vetmeduni Vienna:

Mann-Selberherr Evelyne
Metzler-Zebeli Barbara
Ritzmann Mathias
Schmitz-Esser Stephan
Wagner Martin
Zebeli Qendrim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology
University Clinic for Swine


Project(s): Preventive Veterinary Medicine


Abstract:
Dietary composition largely influences pig"s gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. In the present study, we characterized the mucosa-associated bacterial communities at the Pars non-glandularis of the stomach, ileum and colon, and identified shifts in these communities in response to different dietary calcium-phosphorus (Ca-P) contents (100% versus 190% of the Ca and P requirements) in combination with two basal diets (wheat-barley- or corn-based) in weaned pigs. Pyrosequencing of 16S rRNA genes from 93 mucosal samples yielded 447,849 sequences, clustering into 997 operational taxonomic units (OTUs) at 97% similarity level. OTUs were assigned to 198 genera belonging to 14 different phyla. Correlation-based networks revealed strong interactions among OTUs at the various gastrointestinal sites. Our data describe a previously not reported high diversity and species richness at the Pars non-glandularis of the stomach in weaned pigs. Moreover, high versus adequate Ca-P content significantly promoted Lactobacillus by 14.9% units (1.4 fold change) at the gastric Pars non-glandularis (Pā€Š=ā€Š0.035). Discriminant analysis revealed dynamic changes in OTU composition in response to dietary cereals and Ca-P contents at all gastrointestinal sites which were less distinguishable at higher taxonomic levels. Overall, this study revealed a distinct mucosa-associated bacterial community at the different gut sites, and a strong effect of high Ca-P diets on the gastric community, thereby markedly expanding our comprehension on mucosa-associated microbiota and their diet-related dynamics in weaned pigs.

Keywords Pubmed: Animals
Calcium, Dietary/administration & dosage
Calcium, Dietary/analysis*
Edible Grain/chemistry*
Gastrointestinal Tract/metabolism*
Gastrointestinal Tract/microbiology*
Microbiota*
Mucous Membrane/metabolism
Mucous Membrane/microbiology*
Phosphorus, Dietary/administration & dosage
Phosphorus, Dietary/analysis*
RNA, Ribosomal, 16S/genetics*
Swine
Weaning


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