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Publication type: Journal Article
Document type: Full Paper

Year: 2014

Author(s): Mann, E; Schmitz-Esser, S; Zebeli, Q; Wagner, M; Ritzmann, M; Metzler-Zebeli, BU

Title: Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus.

Source: PLoS One. 2014; 9(1):e86950



Authors Vetmeduni Vienna:

Mann-Selberherr Evelyne
Metzler-Zebeli Barbara
Ritzmann Mathias
Schmitz-Esser Stephan
Wagner Martin
Zebeli Qendrim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
Unit of Food Microbiology
University Clinic for Swine


Project(s): Preventive Veterinary Medicine


Abstract:
Dietary composition largely influences pig"s gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. In the present study, we characterized the mucosa-associated bacterial communities at the Pars non-glandularis of the stomach, ileum and colon, and identified shifts in these communities in response to different dietary calcium-phosphorus (Ca-P) contents (100% versus 190% of the Ca and P requirements) in combination with two basal diets (wheat-barley- or corn-based) in weaned pigs. Pyrosequencing of 16S rRNA genes from 93 mucosal samples yielded 447,849 sequences, clustering into 997 operational taxonomic units (OTUs) at 97% similarity level. OTUs were assigned to 198 genera belonging to 14 different phyla. Correlation-based networks revealed strong interactions among OTUs at the various gastrointestinal sites. Our data describe a previously not reported high diversity and species richness at the Pars non-glandularis of the stomach in weaned pigs. Moreover, high versus adequate Ca-P content significantly promoted Lactobacillus by 14.9% units (1.4 fold change) at the gastric Pars non-glandularis (Pā€Š=ā€Š0.035). Discriminant analysis revealed dynamic changes in OTU composition in response to dietary cereals and Ca-P contents at all gastrointestinal sites which were less distinguishable at higher taxonomic levels. Overall, this study revealed a distinct mucosa-associated bacterial community at the different gut sites, and a strong effect of high Ca-P diets on the gastric community, thereby markedly expanding our comprehension on mucosa-associated microbiota and their diet-related dynamics in weaned pigs.

Keywords Pubmed: Animals
Calcium, Dietary/administration & dosage
Calcium, Dietary/analysis*
Edible Grain/chemistry*
Gastrointestinal Tract/metabolism*
Gastrointestinal Tract/microbiology*
Microbiota*
Mucous Membrane/metabolism
Mucous Membrane/microbiology*
Phosphorus, Dietary/administration & dosage
Phosphorus, Dietary/analysis*
RNA, Ribosomal, 16S/genetics*
Swine
Weaning


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