University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Stalder, GL; Loncaric, I; Walzer, C

Title: Diversity of enterobacteria including β-lactamase producing isolates associated with the Spanish slug (Arion vulgaris).

Source: Sci Total Environ. 2014; 479-480:11-16



Authors Vetmeduni Vienna:

Loncaric Igor
Stalder Gabrielle
Walzer Christian

Vetmed Research Units
Institute of Microbiology
Research Institute of Wildlife Ecology


Abstract:
The aim of this study was to determine diversity in members of the family Enterobacteriaceae including the beta-lactamase producing isolates associated with the Spanish slug (Arion vulgaris). 81 isolates, each representing a distinct colonial morphotype, were obtained from fecal samples of Spanish slugs and analyzed. Genetically heterogeneous or similar groups were assessed among the isolates by enterobacterial repetitive intergenic consensus (ERIC)-PCR and further characterized by 16S rDNA sequence analysis. Representative strains were allocated to the genera Citrobacter, Raoultella, Escherichia, Klebsiella, Kluyvera, and Buttiauxella. Fifteen isolates, most closely related to Citrobacter freundii and Escherichia coli, displayed an AmpC phenotype, one E. coli isolate showed an ESBL phenotype. These isolates were phenotypically and genotypically characterized by their antimicrobial resistance pattern. Phylogenetic background and multi-locus sequence typing (MLST) of the E. coli isolates were determined. The multi-drug resistant TEM-1 E. coli positive isolate belongs to the phylogenetic group A and ST10. The CMY-2 positive E. coli isolate belongs to the phylogenetic group D and ST117. Our results show that the common garden slug represents an important potential vector of β-lactamase producing Enterobacteriaceae.

Keywords Pubmed: Animals
Enterobacteriaceae/classification*
Enterobacteriaceae/genetics
Enterobacteriaceae/metabolism
Gastropoda/microbiology*
Genetic Variation*
Phylogeny
beta-Lactamases/metabolism*


© University of Veterinary Medicine ViennaHelp and Downloads