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Type of publication: Journal Article
Type of document: Full Paper

Year: 2015

Authors: Awad, WA; Molnár, A; Aschenbach, JR; Ghareeb, K; Khayal, B; Hess, C; Liebhart, D; Dublecz, K; Hess, M

Title: Campylobacter infection in chickens modulates the intestinal epithelial barrier function.

Source: Innate Immun. 2015; 21(2):151-160

Authors Vetmeduni Vienna:

Awad Wageha
Ghareeb Khaled
Hess Claudia
Hess Michael
Khayal Basel
Liebhart Dieter

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine
Institute of Animal Nutrition and Functional Plant Compounds

Asymptomatic carriage of Campylobacter jejuni is highly prevalent in chicken flocks. Thus, we investigated whether chronic Campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. An experiment was carried out in which commercial chickens were orally infected with C. jejuni (1 × 10(8) CFU/bird) at 14 days of life. Changes in ion transport and barrier function were assessed by short-circuit current (I(sc)) and transepithelial ion conductance (G(t)) in Ussing chambers. G(t) increased in cecum and colon of Campylobacter-infected chicken 7 d post-infection (DPI), whereas G t initially decreased in the jejunum at 7 DPI and increased thereafter at 14 DPI. The net charge transfer across the epithelium was reduced or tended to be reduced in all segments, as evidenced by a decreased I sc. Furthermore, the infection induced intestinal histomorphological changes, most prominently including a decrease in villus height, crypt depth and villus surface area in the jejunum at 7 DPI. Furthermore, body mass gain was decreased by Campylobacter carriage. This study demonstrates, for the first time, changes in the intestinal barrier function in Campylobacter-infected chickens and these changes were associated with a decrease in growth performance in otherwise healthy-appearing birds.

Keywords Pubmed: Animals
Asymptomatic Diseases
Body Weight
Campylobacter Infections/physiopathology*
Campylobacter jejuni/physiology*
Carrier State
Intestinal Mucosa/microbiology
Intestinal Mucosa/pathology
Intestinal Mucosa/physiology*
Ion Transport/physiology

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