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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2014

Author(s): Sedlak, C; Patzl, M; Saalmüller, A; Gerner, W

Title: CD2 and CD8α define porcine γδ T cells with distinct cytokine production profiles.

Source: Dev Comp Immunol. 2014; 45(1):97-106



Authors Vetmeduni Vienna:

Gerner Wilhelm
Patzl Martina
Saalmüller Armin
Sedlak Corinna

Vetmed Research Units
Institute of Immunology


Abstract:
γδ T cells are a remarkably prominent T-cell subset in swine with a high prevalence in blood. Phenotypic analyses in this study showed that CD2(-) γδ T cells in their vast majority had a CD8α(-)SLA-DR(-)CD27(+) phenotype. CD2(+) γδ T cells dominated in spleen and lymph nodes and had a more heterogeneous phenotype. CD8α(+)SLA-DR(-)CD27(+) γδ T cells prevailed in blood, spleen and lymph nodes whereas in liver a CD8α(+)SLA-DR(+)CD27(-) phenotype dominated, indicating an enrichment of terminally differentiated γδ T cells. γδ T cells were also investigated for their potential to produce IFN-γ, TNF-α and IL-17A. Within CD2(+) γδ T cells, IFN-γ and TNF-α single-producers as well as IFN-γ/TNF-α double-producers dominated, which had a CD8α(+)CD27(+/-) phenotype. IL-17A-producing γδ T cells were only found within CD2(-) γδ T cells, mostly co-produced TNF-α and had a rare CD8α(+)CD27(-) phenotype. However, quantitatively TNF-α single-producers strongly dominated within CD2(-) γδ T cells. In summary, our data identify CD2 and CD8α as important molecules correlating with functional differentiation.

Keywords Pubmed: Animals
Antigens, CD2/metabolism*
Antigens, CD8/metabolism*
Cells, Cultured
Cytokines/metabolism*
Liver/cytology
Lymph Nodes/cytology
Organ Specificity
Spleen/cytology
Sus scrofa/anatomy & histology
Sus scrofa/metabolism*
T-Lymphocytes/metabolism*
Thymus Gland/cytology


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