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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Marco-Ramell, A; Miller, I; Nöbauer, K; Möginger, U; Segalés, J; Razzazi-Fazeli, E; Kolarich, D; Bassols, A

Title: Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection.

Source: J Proteomics. 2014; 101:205-216



Authors Vetmeduni Vienna:

Miller Ingrid
Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute for Medical Biochemistry
VetCore


Abstract:
Haptoglobin (Hp) and immunoglobulins are plasma glycoproteins involved in the immune reaction of the organism after infection and/or inflammation. Porcine circovirus type 2-systemic disease (PCV2-SD), formerly known as postweaning multisystemic wasting syndrome (PMWS), is a globally spread pig disease of great economic impact. PCV2-SD affects the immunological system of pigs causing immunosuppression. The aim of this work was to characterize the Hp protein species of healthy and PCV2-SD affected pigs, as well as the protein backbone and the glycan chain composition of porcine Hp. PCV2-SD affected pigs had an increased overall Hp level, but it did not affect the ratio between Hp species. Glycoproteomic analysis of the Hp β subunits confirmed that porcine Hp is N-glycosylated and, unexpectedly, O-glycosylated, a PTM that is not found on Hp from healthy humans. The glyco-profile of porcine IgG and IgA heavy chains was also characterized; decreased levels of both proteins were found in the investigated group of PCV2-SD affected pigs. Obtained results indicate that no significant changes in the N- and O-glycosylation patterns of these major porcine plasma glycoproteins were detectable between healthy and PCV2-SD affected animals.

Keywords Pubmed: Animals
Circoviridae Infections/immunology
Circoviridae Infections/metabolism*
Circovirus/pathogenicity*
Glycosylation
Haptoglobins/analysis
Haptoglobins/metabolism*
Immunoglobulin A/metabolism*
Immunoglobulin G/metabolism*
Proteome/analysis
Proteome/metabolism
Proteomics
Swine*/immunology
Swine*/metabolism
Swine Diseases/immunology
Swine Diseases/metabolism
Tissue Distribution


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