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Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Martins, NE; Faria, VG; Nolte, V; Schlötterer, C; Teixeira, L; Sucena, É; Magalhães, S

Title: Host adaptation to viruses relies on few genes with different cross-resistance properties.

Source: Proc Natl Acad Sci U S A. 2014; 111(16):5938-5943



Authors Vetmeduni Vienna:

Nolte Viola
Schlötterer Christian

Vetmed Research Units
Institute of Population Genetics


Project(s): Local selective sweeps in Drosophila melanogaster

NGS Speed Mapping


Abstract:
Host adaptation to one parasite may affect its response to others. However, the genetics of these direct and correlated responses remains poorly studied. The overlap between these responses is instrumental for the understanding of host evolution in multiparasite environments. We determined the genetic and phenotypic changes underlying adaptation of Drosophila melanogaster to Drosophila C virus (DCV). Within 20 generations, flies selected with DCV showed increased survival after DCV infection, but also after cricket paralysis virus (CrPV) and flock house virus (FHV) infection. Whole-genome sequencing identified two regions of significant differentiation among treatments, from which candidate genes were functionally tested with RNAi. Three genes were validated--pastrel, a known DCV-response gene, and two other loci, Ubc-E2H and CG8492. Knockdown of Ubc-E2H and pastrel also led to increased sensitivity to CrPV, whereas knockdown of CG8492 increased susceptibility to FHV infection. Therefore, Drosophila adaptation to DCV relies on few major genes, each with different cross-resistance properties, conferring host resistance to several parasites.

Keywords Pubmed: Adaptation, Physiological/genetics*
Adaptation, Physiological/immunology
Animals
Disease Resistance/genetics*
Disease Resistance/immunology
Drosophila melanogaster/genetics*
Drosophila melanogaster/immunology
Drosophila melanogaster/virology*
Gene Knockdown Techniques
Genes, Insect/genetics*
Genetic Association Studies
Host-Pathogen Interactions/genetics
Host-Pathogen Interactions/immunology*
Insect Viruses/immunology*
Parasites/immunology
RNA Interference
Reproducibility of Results
Selection, Genetic
Species Specificity
Virus Diseases/genetics
Virus Diseases/immunology
Virus Diseases/virology


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