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Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Menanteau-Ledouble, S; Kattlun, J; Nöbauer, K; El-Matbouli, M

Title: Protein expression and transcription profiles of three strains of Aeromonas salmonicida ssp. salmonicida under normal and iron-limited culture conditions.

Source: Proteome Sci. 2014; 12:29



Authors Vetmeduni Vienna:

El-Matbouli Mansour
Kattlun Julia
Menanteau-Ledouble Simon
Nöbauer Katharina

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Fish Medicine
VetCore


Project(s): In-vivo induzierte Antigen-Technologie von Aeromonas salmonicida


Abstract:
Aeromonas salmonicida is an important fish pathogen that produces a wide and varied array of virulence factors. Here we used iron deprivation by addition of the chelator 2"2-dipyridyl to induce the expression of several such virulence factors in three isolates of Aeromonas salmonicida (one avirulent and two virulent). By using SDS-PAGE followed by mass spectrometry, we identified proteins that appeared differentially expressed under these conditions. The differential transcription of the identified gene products were subsequently measured by reverse transcription quantitative real-time PCR (RT-qPCR).


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