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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2014

Author(s): Rütgen, BC; Gerner, W; Beiglböck, C; Schaschl, H; Saalmüller, A; Suchentrunk, F; Essler, SE

Title: Exploratory assessment of CD4+ T lymphocytes in brown hares (Lepus europeus) using a cross-reactive anti-rabbit CD4 antibody.

Source: Vet Immunol Immunopathol. 2014; 161(1-2):108-115



Authors Vetmeduni Vienna:

Beiglböck Christoph
Gerner Wilhelm
Hammer Sabina
Rütgen Barbara
Saalmüller Armin
Suchentrunk Franz

Vetmed Research Units
Institute of Immunology
Research Institute of Wildlife Ecology


Project(s): MHC variability, endoparasites and fitness in hares


Abstract:
In lagomorphs, lymphocyte subset distributions and the importance of CD4(+) T cell levels has so far only been considered in the frame of rabbit disease models. In this study, the first assessment of CD4(+) T lymphocytes in peripheral blood cells in brown hares (Lepus europaeus L., 1758), a further leporid species using a cross-reactive rabbit anti-CD4 antibody in flow cytometry, is presented. In addition, the entire coding region of the hare CD4 gene (1380 bp) coding for a polypeptide of 459 amino acids has been sequenced. Using generalized least squares fitting by maximum likelihood (GLS) test, significantly (p=0.0095) higher CD4(+) T cell frequencies in males than in females and significantly (p=0.0001) higher frequencies for leverets (younger than 2 months of age) than for subadult and adult (older than 7 months of age) individuals were detected. No significant age influence, however, was found for subadult and adult hares. The study is particularly meant to provide a first step in establishing a toolbox for the assessment of the immune response in this leporid species.

Keywords Pubmed: Amino Acid Sequence
Animals
Antibodies
Antigens, CD4/genetics
Antigens, CD4/metabolism
CD4-Positive T-Lymphocytes/cytology
CD4-Positive T-Lymphocytes/immunology*
CD4-Positive T-Lymphocytes/physiology*
Female
Hares/immunology*
Male
Molecular Sequence Data
Rabbits
Species Specificity
Spleen/cytology


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