University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Open Access Logo

Type of publication: Journal Article
Type of document: Review Article

Year: 2015

Authors: Schlötterer, C; Kofler, R; Versace, E; Tobler, R; Franssen, SU

Title: Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.

Source: Heredity (Edinb). 2015; 114(5):431-440



Authors Vetmeduni Vienna:

Franssen Susanne
Kofler Robert
Schlötterer Christian
Tobler Raymond
Versace Elisabetta

Vetmed Research Units
Institute of Population Genetics


Project(s): ERC ADG: The architecture of adaptation


Abstract:
Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.

Keywords Pubmed: Adaptation, Physiological/genetics*
Animals
Biological Evolution*
Drosophila melanogaster/genetics
Gene Frequency
Genetic Association Studies
Genetic Variation*
Genotype
High-Throughput Nucleotide Sequencing*
Phenotype
Polymorphism, Single Nucleotide
Research Design


© University of Veterinary Medicine ViennaHelp and DownloadsAccessibility statement