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Selected Publication:

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Type of publication: Journal Article
Type of document: Review Article

Year: 2015

Authors: Schlötterer, C; Kofler, R; Versace, E; Tobler, R; Franssen, SU

Title: Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.

Source: Heredity (Edinb). 2015; 114(5):431-440

Authors Vetmeduni Vienna:

Franssen Susanne
Kofler Robert
Schlötterer Christian
Tobler Raymond
Versace Elisabetta

Vetmed Research Units
Institute of Population Genetics

Project(s): ERC ADG: The architecture of adaptation

Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.

Keywords Pubmed: Adaptation, Physiological/genetics*
Biological Evolution*
Drosophila melanogaster/genetics
Gene Frequency
Genetic Association Studies
Genetic Variation*
High-Throughput Nucleotide Sequencing*
Polymorphism, Single Nucleotide
Research Design

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