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Selected Publication:

Type of publication: Journal Article
Type of document: Letter

Year: 2014

Authors: Gannoun-Zaki, L; Belon, C; Dupont, C; Hilbert, F; Kremer, L; Blanc-Potard, AB

Title: Overexpression of the Salmonella KdpF membrane peptide modulates expression of kdp genes and intramacrophage growth.

Source: FEMS Microbiol Lett. 2014; 359(1):34-41



Authors Vetmeduni Vienna:

Hilbert Friederike

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Hygiene and Technology


Abstract:
Membrane peptides appear as an emerging class of regulatory molecules in bacteria, which can interact with membrane proteins, including transporters and sensor kinases. The KdpF peptide, which is cotranscribed with kdpABC genes and regulated by the KdpDE two-component system, is supposed to stabilize the KdpABC potassium transporter complex but may also exhibit unsuspected regulatory function(s). The mycobacterial KdpF can interact with the KdpD histidine kinase, and kdpF overexpression has been shown to reduce intramacrophage replication of Mycobacterium bovis BCG. In this study, we investigated whether KdpF displays similar behavior in another intracellular pathogen, Salmonella enterica serovar Typhimurium. We show that Salmonella KdpF can interact with KdpD in a bacterial two-hybrid assay. We have constructed a Salmonella strain overexpressing kdpF, and we have investigated expression of the kdp regulon, as well as intramacrophage survival. We show that kdpF overexpression reduces expression of kdpA and kdpD genes under potassium limitation. Moreover, kdpF overexpression increases intramacrophage multiplication of S. Typhimurium. Hence, our results indicate that KdpF can play a regulatory role in S. Typhimurium, modulating kdp gene expression and intramacrophage survival, but in a way that differs from the one reported for M. bovis BCG.

Keywords Pubmed: Adenosine Triphosphatases/genetics
Adenosine Triphosphatases/metabolism*
Bacterial Proteins/genetics
Bacterial Proteins/metabolism*
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Macrophages/microbiology*
Membrane Proteins/metabolism*
Microbial Viability
Protein Interaction Mapping
Protein Kinases/metabolism
Salmonella typhimurium/genetics
Salmonella typhimurium/growth & development*
Salmonella typhimurium/metabolism*
Two-Hybrid System Techniques
Virulence Factors/genetics
Virulence Factors/metabolism*


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