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Selected Publication:

Type of publication: Journal Article
Type of document: Note

Year: 2015

Authors: Steinberger, B; Brem, G; Mayrhofer, C

Title: Evaluation of SYPRO Ruby total protein stain for the normalization of two-dimensional Western blots.

Source: Anal Biochem. 2015; 476:17-19

Authors Vetmeduni Vienna:

Brem Gottfried
Itze-Mayrhofer Corina
Steinberger Birgit

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology

Due to post-translational modifications such as phosphorylation, proteins exist as distinct charge variants. Two-dimensional (2D) gel electrophoresis followed by immunoblotting enables the detection of these isoforms. For their accurate relative quantitation in different samples, a loading control is necessary to compensate for technical errors such as imprecise sample loading or transfer. The study reveals that the combinatory approach of SYPRO Ruby and chemiluminescence-based 2D Western blot analysis exhibits high linearity and excellent reproducibility and is applicable for limited sample amounts.

Keywords Pubmed: Blotting, Western
Electrophoresis, Gel, Two-Dimensional/methods*
Organometallic Compounds/chemistry*

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