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Publication type: Journal Article
Document type: Full Paper

Year: 2015

Author(s): Rodríguez-Gómez, IM; Käser, T; Gómez-Laguna, J; Lamp, B; Sinn, L; Rümenapf, T; Carrasco, L; Saalmüller, A; Gerner, W

Title: PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate.

Source: Vet Res. 2015; 46:54



Authors Vetmeduni Vienna:

Gerner Wilhelm
Käser Tobias
Lamp Benjamin
Rodriguez Gomez Irene Magdalena
Rümenapf Hans Tillmann
Saalmüller Armin
Sinn Leonie

Vetmed Research Units
Institute of Immunology
Institute of Virology


Abstract:
In vitro generated monocyte-derived dendritic cells (moDCs) have frequently been used to study the influence of porcine reproductive and respiratory syndrome virus (PRRSV) infection on antigen presenting cells. However, obtained results have often been conflicting in regard to expression of co-stimulatory molecules and interaction with T cells. In this study we performed a detailed phenotypic characterisation of PRRSV-infected moDCs and non-infected moDCs. For CD163 and CD169, which are involved in PRRSV-entry into host cells, our results show that prior to infection porcine moDCs express high levels of CD163 but only very low levels for CD169. Following infection with either PRRSV-1 or PRRSV-2 strains after 24 h, PRRSV-nucleoprotein (N-protein)(+) and N-protein(-) moDCs derived from the same microculture were analyzed for expression of swine leukocyte antigen-DR (SLA-DR) and CD80/86. N-protein(+) moDCs consistently expressed higher levels of SLA-DR and CD80/86 compared to N-protein(-) moDCs. We also investigated the influence of PRRSV-infected moDCs on proliferation and frequency of Foxp3(+) regulatory T cells present within CD4(+) T cells in in vitro co-cultures. Neither CD3-stimulated nor unstimulated CD4(+) T cells showed differences in regard to proliferation and frequency of Foxp3(+) T cells following co-cultivation with either PRRSV-1 or PRRSV-2 infected moDCs. Our results suggest that a more detailed characterisation of PRRSV-infected moDCs will lead to more consistent results across different laboratories and PRRSV strains as indicated by the major differences in SLA-DR and CD80/86 expression between PRRSV-infected and non-infected moDCs present in the same microculture.

Keywords Pubmed: Animals
Antigens, CD80/immunology
Antigens, CD80/metabolism
Antigens, CD86/immunology
Antigens, CD86/metabolism
Dendritic Cells/immunology
Dendritic Cells/metabolism
Forkhead Transcription Factors/genetics*
Forkhead Transcription Factors/metabolism
Histocompatibility Antigens Class II/immunology
Histocompatibility Antigens Class II/metabolism
Monocytes/metabolism
Porcine Reproductive and Respiratory Syndrome/immunology*
Porcine Reproductive and Respiratory Syndrome/virology
Porcine respiratory and reproductive syndrome virus/physiology*
Swine
T-Lymphocytes, Regulatory/immunology
T-Lymphocytes, Regulatory/metabolism


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