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Type of publication: Journal Article
Type of document: Full Paper

Year: 2015

Authors: Maillo, V; Gaora, PÓ; Forde, N; Besenfelder, U; Havlicek, V; Burns, GW; Spencer, TE; Gutierrez-Adan, A; Lonergan, P; Rizos, D

Title: Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?

Source: Biol Reprod. 2015; 92(6):144

Authors Vetmeduni Vienna:

Besenfelder Urban
Havlicek Vitezslav

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology

This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In experiment 2, heifers were divided into cyclic (nonbred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 postestrus (n = 50 zygotes/heifer). Heifers were slaughtered on Day 3, and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes, of which 123 were up-regulated and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. In conclusion, the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes in the oviductal isthmus; failure to detect changes in the oviduct transcriptome in the presence of a single embryo may be due to the effect being local and undetectable under the conditions of this study.

Keywords Pubmed: Animals
Embryo, Mammalian/metabolism
Embryo, Mammalian/physiology*
Gene Expression Profiling
Gene Expression Regulation
Tissue Array Analysis

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