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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1996

Author(s): Kühholzer, B; Müller, S; Besenfelder, U; Brem, G

Title: Endoscopic Embryo Transfer Techniques In Sheep .2. Endoscopic Ovum Pick Up.

Source: Wien Tierarztl Monat. 1996; 83(12): 370-373.

Authors Vetmeduni Vienna:

Besenfelder Urban
Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Besides embryo flushings ex vivo ovum pick up presents an alternative technique for embryo production. Recovered cumulus-oocyte-complexes are the basis for in vitro production of embryos. Endoscopic ovum pick ups were performed in hormonally untreated ewes. The aspiration of the follicular fluid was carried out with a 5 mi syringe combined with a 0.9 mm canula. Alltogether 134 ewes served as ovum pick up-donors. An average of 5.7 follicles per animal were punctured and 58% of the oocytes were collected. In this study a simple method for endoscopic oocyte aspiration was described. The technique used allows the recovery of a high percentage of oocytes of hormonally untreated ewes and gives the possibility to produce a high number of embryos without superovulation of the donors.


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