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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1995

Author(s): Schellander, K; Peli, J; Schmoll, F; Brem, G

Title: Artificial Insemination In Cattle With Dna-treated Sperm.

Source: Anim Biotechnol (6), 1 41-50.



Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
We have investigated the use of sperm cells as vectors for transferring exogenous DNA into the genome of cattle by artificial insemination with DNA-treated sperm. First we demonstrated the DNA-binding ability of cattle sperm with radioactively labeled DNA. For artificial insemination ejaculated semen was washed and incubated with 1 mu g DNA/10(6) sperm for one hour at 37 degrees C. Three hundred synchronized heifers were inseminated once with a dose of 40x10(6) sperm. Forty-five calves and 41 fetuses were obtained. Southern analysis revealed in one calf a signal after probing with the 1 kb Pst I fragment of pSV2-cat.


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