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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1995

Author(s): Schellander, K; Peli, J; Schmoll, F; Brem, G

Title: Artificial Insemination In Cattle With Dna-treated Sperm.

Source: Anim Biotechnol (6), 1 41-50.

Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics

We have investigated the use of sperm cells as vectors for transferring exogenous DNA into the genome of cattle by artificial insemination with DNA-treated sperm. First we demonstrated the DNA-binding ability of cattle sperm with radioactively labeled DNA. For artificial insemination ejaculated semen was washed and incubated with 1 mu g DNA/10(6) sperm for one hour at 37 degrees C. Three hundred synchronized heifers were inseminated once with a dose of 40x10(6) sperm. Forty-five calves and 41 fetuses were obtained. Southern analysis revealed in one calf a signal after probing with the 1 kb Pst I fragment of pSV2-cat.

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