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Publication type: Journal Article
Document type: Full Paper

Year: 2015

Author(s): Spiesberger, K; Paulfranz, F; Egger, A; Reiser, J; Vogl, C; Rudolf-Scholik, J; Mayrhofer, C; Grosse-Hovest, L; Brem, G

Title: Large-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle.

Source: PLoS One. 2015; 10(10):e0140471



Authors Vetmeduni Vienna:

Brem Gottfried
Itze-Mayrhofer Corina
Spiesberger Katrin
Vogl Claus

Vetmed Research Units
Unit of Molecular Genetics
Unit of Reproductive Biology


Abstract:
30 years ago, the potential of bispecific antibodies to engage cytotoxic T cells for the lysis of cancer cells was discovered. Today a variety of bispecific antibodies against diverse cell surface structures have been developed, the majority of them produced in mammalian cell culture systems. Beside the r28M, described here, no such bispecific antibody is known to be expressed by transgenic livestock, although various biologicals for medical needs are already harvested-mostly from the milk-of these transgenics. In this study we investigated the large-scale purification and biological activity of the bispecific antibody r28M, expressed in the blood of transgenic cattle. This tandem single-chain variable fragment antibody is designed to target human CD28 and the melanoma/glioblastoma-associated cell surface chondroitin sulfate proteoglycan 4 (CSPG4).

Keywords Pubmed: Adult
Animals
Animals, Genetically Modified
Antibodies, Bispecific/immunology*
Antigens, CD28/immunology
Cattle/genetics
Cattle/immunology*
Cell Line, Tumor
Chondroitin Sulfate Proteoglycans/immunology*
Humans
Male
Melanoma/immunology*
Membrane Proteins/immunology*
Single-Chain Antibodies/immunology*


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