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Type of publication: Journal Article
Type of document: Full Paper

Year: 2004

Authors: Neubauer, C; Szölgyenyi, W; Jauk, V; Vasicek, L

Title: Polymerase chain reaction versus culture technique for detection of thermophilic Campylobacter in chicken faecal samples.

Source: Veterinary Medicine Austria / Wiener Tierärztliche Monatsschrift (91) 287-291.

Authors Vetmeduni Vienna:

Hess Claudia
Vasicek Lore

Vetmed Research Units
University Clinic for Poultry and Fish Medicine

The suitability of a polymerase chain reaction (PCR) as a diagnostic method for detection of Campylobacter sp. in chicken faecal samples was tested. The primers CF171 and CR522 used in this study were designed to amplify the 16S rRNA gene of Campylobacter. Reference strains from the National Collection of Type Cultures (NCTC), London, and 120 culture-isolates identified as thermophilic Campylobacter by phenotypic tests as well as by restriction fragment length polymorphism of PCR product (PCR-RFLP), were investigated with this PCR. Using PCR, 138 chicken faecal samples were directly examined for the presence of Campylobacter. Results were compared with those obtained from the analysis of the same samples by culture technique. PCR was found to detect more positive samples than culture method. Therefore PCR seems to be a more sensitive method to detect Campylobactersp. compared to the culture technique although an exact assessment of the diagnostic sensitivity and specificity due to the lack of a defined gold standard was not possible.

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