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Selected Publication:

Type of publication: Journal Article
Type of document: Report

Year: 2016

Authors: Wu, J; Stefaniak, J; Hafner, C; Schramel, JP; Kaun, C; Wojta, J; Ullrich, R; Tretter, VE; Markstaller, K; Klein, KU

Title: Intermittent Hypoxia Causes Inflammation and Injury to Human Adult Cardiac Myocytes.

Source: Anesth Analg. 2016; 122(2):373-380

Authors Vetmeduni Vienna:

Schramel Johannes

Vetmed Research Units
University Clinic for Small Animals, Clinical Unit of Anaesthesiology and Perioperative Intensive-Care Medicine

Intermittent hypoxia may occur in a number of clinical scenarios, including interruption of myocardial blood flow or breathing disorders such as obstructive sleep apnea. Although intermittent hypoxia has been linked to cardiovascular and cerebrovascular disease, the effect of intermittent hypoxia on the human heart is not fully understood. Therefore, in the present study, we compared the cellular responses of cultured human adult cardiac myocytes (HACMs) exposed to intermittent hypoxia and different conditions of continuous hypoxia and normoxia.HACMs were exposed to intermittent hypoxia (0%-21% O2), constant mild hypoxia (10% O2), constant severe hypoxia (0% O2), or constant normoxia (21% O2), using a novel cell culture bioreactor with gas-permeable membranes. Cell proliferation, lactate dehydrogenase release, vascular endothelial growth factor release, and cytokine (interleukin [IL] and macrophage migration inhibitory factor) release were assessed at baseline and after 8, 24, and 72 hours of exposure. A signal transduction pathway finder array was performed to determine the changes in gene expression.In comparison with constant normoxia and constant mild hypoxia, intermittent hypoxia induced earlier and greater inflammatory response and extent of cell injury as evidenced by lower cell numbers and higher lactate dehydrogenase, vascular endothelial growth factor, and proinflammatory cytokine (IL-1β, IL-6, IL-8, and macrophage migration inhibitory factor) release. Constant severe hypoxia showed more detrimental effects on HACMs at later time points. Pathway analysis demonstrated that intermittent hypoxia primarily altered gene expression in oxidative stress, Wnt, Notch, and hypoxia pathways.Intermittent and constant severe hypoxia, but not constant mild hypoxia or normoxia, induced inflammation and cell injury in HACMs. Cell injury occurred earliest and was greatest after intermittent hypoxia exposure. Our in vitro findings suggest that intermittent hypoxia exposure may produce rapid and substantial damage to the human heart.

Keywords Pubmed: Adult
Cell Proliferation
Cells, Cultured
Gene Expression
L-Lactate Dehydrogenasemetabolism
Membranes, Artificial
Myocytes, Cardiacpathology
Signal Transduction
Sleep Apnea, Obstructivepathology
Vascular Endothelial Growth Factor Ametabolism

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