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Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Loncaric, I; Beiglböck, C; Feßler, AT; Posautz, A; Rosengarten, R; Walzer, C; Ehricht, R; Monecke, S; Schwarz, S; Spergser, J; Kübber-Heiss, A

Title: Characterization of ESBL- and AmpC-Producing and Fluoroquinolone-Resistant Enterobacteriaceae Isolated from Mouflons (Ovis orientalis musimon) in Austria and Germany.

Source: PLoS One. 2016; 11(5):e0155786



Authors Vetmeduni Vienna:

Beiglböck Christoph
Kübber-Heiss Anna
Loncaric Igor
Posautz Annika
Rosengarten Renate
Spergser Joachim
Walzer Christian

Vetmed Research Units
Research Institute of Wildlife Ecology, Conservation Medicine
Institute of Microbiology
Research Institute of Wildlife Ecology


Abstract:
The aim of this study was to investigate the presence of β-lactamase producing or fluoroquinolone-resistant members of the family Enterobacteriaceae in European mouflons (Ovis orientalis musimon). The mouflon samples originated from nasal and perineal swabs and/or organ samples in cases of a suspected infection. Only one of the 32 mouflons was tested positive for the presence of Enterobacteriaceae that displayed either an ESBL/AmpC phenotype or were resistant to ciprofloxacin. The positively tested swab originated from a sample of the jejunal mucosa of a four-year old female mouflon. Two different colony morphotypes were identified as Escherichia coli and Klebsiella pneumoniae. These isolates were phenotypically and genotypically characterized in detail by a polyphasic approach. Both isolates were multi-drug resistant. The E. coli isolate belonged to the phylogenetic group B1 and sequence type (ST) 744 and harboured the β-lactamase genes blaCTX-M-15 and blaOXA-1. The K. pneumoniae, identified as ST11, harboured the β-lactamase genes blaSHV-11, blaOXA-1, and blaDHA-1 as well as the plasmid-mediated quinolone resistance (PMQR) gene qnrB55. The present study demonstrates that wild animals can acquire human-derived resistance determinants and such findings may indicate environmental pollution with resistance determinants from other sources.

Keywords Pubmed: Animals
Anti-Bacterial Agents/pharmacology*
Bacterial Proteins/genetics
Bacterial Proteins/metabolism*
Drug Resistance, Bacterial*
Enterobacteriaceae/drug effects
Enterobacteriaceae/enzymology*
Enterobacteriaceae/genetics
Enterobacteriaceae/isolation & purification
Female
Gastrointestinal Microbiome
Jejunum/microbiology
Quinolones/pharmacology*
Sheep/microbiology
beta-Lactamases/genetics
beta-Lactamases/metabolism*


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