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Selected Publication:

Type of publication: Journal Article
Type of document: Short Communication

Year: 2016

Authors: Schön K; Schornsteiner E; Dzieciol M; Wagner M; Müller M; Schmitz-Esser S

Title: Microbial communities in dairy processing environment floor-drains are dominated by product-associated bacteria and yeasts.

Source: Food Control 2016; 70: 210-215

Authors Vetmeduni Vienna:

Dzieciol Monika
Müller Mathias
Schmitz-Esser Stephan
Schön Kerstin
Schornsteiner Elisa
Wagner Martin

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology
Institute of Animal Breeding and Genetics, Unit of Molecular Genetics

Project(s): Protection of consumers by microbial risk mitigation through combating segregation of expertise

Jak Stat - Signalling from Basis to Disease

Floor-drains in food, production environments harbour diverse microbes and can be contamination sources. To prevent cross-contamination with spoilage organisms or pathogens, sanitation protocols are regularly applied in food processing facilities. Along with product-associated samples, floor-drain water serves as an important indicator in hygiene monitoring in food processing facilities. However, knowledge about the microbial communities in floor-drains is still low. In this study, the microbial communities in floor-drain water and biofilm samples from an Austrian dairy plant were analysed and revealed that floor-drain microbial communities are dominated by product-associated microbes. DNA was extracted of drain water and drain biofilm samples from three different areas (n = 6) of an Austrian cheese production facility. To characterise the bacterial and eukaryotic communities, 16S rRNA gene pyrosequencing as well as cloning and sequencing of 18S rRNA genes were used. Floor-drain communities were dominated by product-associated bacterial (e.g. Lactobacillus kefiranofaciens, Streptococcus thermophilus) and eukaryotic phylotypes (e.g. Debaryomyces hansenii, Saccharomyces unisporus). In addition, putative drain water derived phylotypes (e.g. Psychrilyobacter atlanticus, Cobetia marina) and ciliates were identified. (C) 2016 Elsevier Ltd. All rights reserved.

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