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Publication type: Journal Article
Document type: Full Paper

Year: 2016

Author(s): Humer, E; Khol-Parisini, A; Metzler-Zebeli, BU; Gruber, L; Zebeli, Q

Title: Alterations of the Lipid Metabolome in Dairy Cows Experiencing Excessive Lipolysis Early Postpartum.

Source: PLoS One. 2016; 11(7):e0158633



Authors Vetmeduni Vienna:

Humer Elke
Khol Annabella
Metzler-Zebeli Barbara
Zebeli Qendrim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
University Clinic for Swine


Project(s): Advancement of Dairying in Austria


Abstract:
A decrease in insulin sensitivity enhances adipose tissue lipolysis helping early lactation cows counteracting their energy deficit. However, excessive lipolysis poses serious health risks for cows, and its underlying mechanisms are not clearly understood. The present study used targeted ESI-LC-MS/MS-based metabolomics and indirect insulin sensitivity measurements to evaluate metabolic alterations in the serum of dairy cows of various parities experiencing variable lipolysis early postpartum. Thirty (12 primiparous and 18 multiparous) cows of Holstein Friesian and Simmental breeds, fed the same diet and kept under the same management conditions, were sampled at d 21 postpartum and classified as low (n = 10), medium (n = 8), and high (n = 12) lipolysis groups, based on serum concentration of nonesterified fatty acids. Overall, excessive lipolysis in the high group came along with impaired estimated insulin sensitivity and characteristic shifts in acylcarnitine, sphingomyelin, phosphatidylcholine and lysophospholipid metabolome profiles compared to the low group. From the detected phosphatidylcholines mainly those with diacyl-residues showed differences among lipolysis groups. Furthermore, more than half of the detected sphingomyelins were increased in cows experiencing high lipomobilization. Additionally, strong differences in serum acylcarnitines were noticed among lipolysis groups. The study suggests an altered serum phospholipidome in dairy cows associated with an increase in certain long-chain sphingomyelins and the progression of disturbed insulin function. In conclusion, the present study revealed 37 key metabolites as part of alterations in the synthesis or breakdown of sphingolipids and phospholipids associated with lowered estimated insulin sensitivity and excessive lipolysis in early-lactating cows.

Keywords Pubmed: Adipose Tissue/metabolism
Animals
Cattle
Chromatography, Liquid/methods
Dairying
Energy Metabolism
Fatty Acids, Nonesterified/blood
Female
Insulin Resistance
Lactation
Lipids/blood*
Lipolysis*
Lysophosphatidylcholines/blood
Metabolome*
Metabolomics/methods*
Milk/metabolism*
Parity
Phosphatidylcholines/blood
Postpartum Period/blood
Sphingomyelins/blood
Tandem Mass Spectrometry/methods


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