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Selected Publication:

Type of publication: Journal Article
Type of document: Short Communication

Year: 2016

Authors: Liu, L; Schwarz, L; Ullman, K; Ahola, H; Qiu, Y; Ma, Z; Hennig-Pauka, I

Title: Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach.

Source: J Gen Virol. 2016; 97(7):1592-1596



Authors Vetmeduni Vienna:

Hennig-Pauka Isabel
Schwarz Lukas

Vetmed Research Units
University Clinic for Swine


Abstract:
Bufavirus is a single-stranded DNA virus belonging to the genus Protoparvovirus. This study reports the identification and characterization of a porcine bufavirus by a metagenomic approach, and a limited epidemiology investigation of bufavirus in six swine farms. A comparative genome analysis showed a similarity of 93 % to a Hungarian porcine bufavirus. Bayesian and maximum-likelihood analyses of genome sequences showed a close relationship of porcine bufaviruses to human and monkey bufaviruses. Molecular dating of the most recent common ancestors supported a recent introduction of bufaviruses into human and pig populations, respectively. A real-time PCR method was developed to screen 60 faecal samples for the porcine bufavirus DNA, and eight positive samples were found in two neighbouring farms, suggesting a relatively low prevalence (13.3 %). No direct transmission of porcine bufaviruses between two neighbouring farms was found, suggesting that bufaviruses may have spread widely in different geographical regions.

Keywords Pubmed: Animals
Base Sequence
DNA, Single-Strandedgenetics
DNA, Viralgenetics
Genome, Viralgenetics
Metagenomicsmethods
Parvoviridae Infectionsvirology
Parvovirinaeclassificationgeneticsisolation & purification
Real-Time Polymerase Chain Reactionmethods
Sequence Analysis, DNA
Sus scrofa
Swine
Swine Diseasesvirology

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