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Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Forde, N; O'Gorman, A; Whelan, H; Duffy, P; O'Hara, L; Kelly, AK; Havlicek, V; Besenfelder, U; Brennan, L; Lonergan, P

Title: Lactation-induced changes in metabolic status and follicular-fluid metabolomic profile in postpartum dairy cows.

Source: Reproduction, Fertility and Development 2016; 28: 1882-1892



Authors Vetmeduni Vienna:

Besenfelder Urban
Havlicek Vitezslav

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology


Abstract:
The aim was to investigate the effect of lactation on the composition of pre-ovulatory follicular fluid (FF). Forty in-calf primiparous heifers and 20 maiden heifers were enrolled. Immediately after calving, half of the cows were dried off while the remainder were milked twice daily. Serum samples were collected twice weekly from two weeks pre- to 84 days postpartum (dpp). FF was analysed by gas chromatography-mass spectrometry. Serum concentrations of non-esterified fatty acids and β-hydroxybutyrate were higher, while glucose, insulin and Insulin-like growth factor 1 (IGF1) concentrations were lower in lactating cows compared with non-lactating cows and heifers (P<0.01). Principal component analysis of FF metabolites revealed a clear separation of the lactating group from both non-lactating cows and heifers. The amino acids tyrosine, phenylalanine and valine and fatty acids heneicosanoic acid and docosahexaenoic acid were all lower in FF from lactating compared with dry cows (P<0.05). FF from lactating cows was higher in aminoadipic acid, α-aminobutyric acid, glycine and serine while histidine, leucine, lysine, methionine and ornithine were all lower than in dry cows and heifers (P<0.05). The ratio of n6:n3 was higher in lactating cows compared with both non-lactating cows and heifers, whereas total n3 polyunsaturated fatty acids, pentadecanoic, linolenic, elaidic and arachidonic acids were all lower in the FF of lactating cows than both non-lactating cows and heifers (P<0.05). In conclusion, lactation induces distinct changes in the overall metabolic status of postpartum lactating dairy cows which are associated with divergent metabolite profiles in FF.

Keywords Pubmed: Animals
Cattlephysiology
Fatty Acids, Nonesterifiedblood
Female
Follicular Fluidmetabolism
Lactation
Metabolome
Postpartum Period

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