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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2005

Author(s): Lopucki, M; Schmerold, I; Dadak, A; Wiktor, H; Niederm├╝ller, H; Kankofer, M

Title: Low dose magnetic fields do not cause oxidative DNA damage in human placental cotyledons in vitro.

Source: Virchows Arch. 2005; 446(6):634-639



Authors Vetmeduni Vienna:

Dadak Agnes
Niederm├╝ller Hans
Schmerold Ivo

Vetmed Research Units
Institute of Pharmacology and Toxicology
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology


Project(s): Investigations to determine oxidative damages of cellular DNA in animal and human organs


Abstract:
The biological impact of low dose magnetic fields generated by electric appliances present in the human environment is still uncertain. In this study, human placentas served as a model tissue for the evaluation of the potential effect of oscillating low intensity magnetic fields on the concentration of 8-hydroxy-2"-deoxyguanosine (8-OH-dG) in cellular DNA. Cotyledons were dissected from placentas obtained immediately after physiological labours and exposed to magnetic fields (groups MF A, 2 mT, 50 Hz and MF B, 5 mT, 50 Hz) or sham exposed (group C) during an in vitro perfusion of 3 h. Cellular DNA was isolated, hydrolyzed and analyzed by HPLC. Native nucleosides were monitored at 254 nm and 8-OH-dG by electrochemical detection. Results were expressed as mumol 8-OH-dG/mol deoxyguanosine (dG). The concentrations of 8-OH-dG in group C, MF A and MF B were 28.45+/-15.27 micromol/mol dG, 62.80+/-31.91 mumol/mol dG, and 27.49+/-14.23 micromol/mol dG, respectively, demonstrating no significant difference between the groups. The results suggest that placental tissues possess a capacity to protect DNA against oxidative alterations by magnetic field of intensities previously shown to produce radical mediated DNA damage in rat brain cells in vivo and imbalances in electrolyte release of cotyledons under in vitro conditions.

Keywords Pubmed: Adolescent
Adult
Chromatography, High Pressure Liquid
DNA Damage/radiation effects*
Deoxyguanosine/analogs & derivatives*
Deoxyguanosine/analysis
Dose-Response Relationship, Radiation
Electromagnetic Fields/adverse effects*
Female
Humans
Oxidative Stress/physiology*
Placenta/radiation effects*
Pregnancy


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