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Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Jaureguiberry, M; Madoz, LV; Giuliodori, MJ; Wagener, K; Prunner, I; Grunert, T; Ehling-Schulz, M; Drillich, M; de la Sota, RL

Title: Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy.

Source: Acta Vet Scand. 2016; 58(1):81

Authors Vetmeduni Vienna:

Drillich Marc
Ehling-Schulz Monika
Grunert Tom
Prunner Isabella
Wagener Karen

Vetmed Research Units
University Clinic for Ruminants, Clinical Unit of Herd Management in ruminants
Institute of Microbiology, Functional Microbiology Division

Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27).

Keywords Pubmed: Actinomycetaceae/classification
Actinomycetaceae/isolation & purification*
Actinomycetales Infections/diagnosis
Actinomycetales Infections/microbiology
Actinomycetales Infections/veterinary*
Animal Husbandry/methods*
Bacteriological Techniques/veterinary
Cattle Diseases/diagnosis
Cattle Diseases/microbiology*
Databases, Factual
Escherichia coli/classification
Escherichia coli/isolation & purification*
Escherichia coli Infections/microbiology
Escherichia coli Infections/veterinary*
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared*
Uterine Diseases/diagnosis
Uterine Diseases/microbiology
Uterine Diseases/veterinary

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