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Publication type: Journal Article
Document type: Full Paper

Year: 1997

Author(s): Muller, K; Kasbohrer, A; Blaha, T

Title: Evaluation of a modification of the ISO 6579 Salmonella detection for its usability for monitoring poultry meat production chains

Source: Fleischwirtschaft (77), 6 563-567.

Authors Vetmeduni Vienna:

Käsbohrer Annemarie

In the framework of a long-term study on the occurence and control of salmonella in a poultry meat production chain, a modification of detecting salmonella according to ISO 6579 was evaluated for its usibiality for monitoring all stages of the poultry meat production. The modification was needed, since the ISO 6579 method was developed exclusively for meat and feedstuffs. Monitoring a meat production chain through all stages, however, demands the salmonella detection, apart from meat and feedstuffs, in diverse materials such as faeces, litter, animal tissues, surface swabs, dust, rodents, water etc. In a preliminary trial, 303 samples were investigated by using simultaneously the ISO 6579 method and its modification. The modification consists of: 1. Pre-enrichment in buffered pepton water (gPW), 2. Selective enrichment in tetrathionate-brillant-green-bile-broth (TBG-broth) and Rappaport-Vassiliadis-broth (RV-broth), and, 3. culturing of the selective enrichment broth on Rambach- and xylose-lysine-desoxycholate-agar (XLD-agar). In the preliminary trial, the TBG-broth showed a remarkable higher sensitivity than the selenit-cystin-broth that is recommended by ISO 6579. Likewise, remarkably more salmonellae were isolated by using the Rambach-agar instead of the brillantgreen-phenolred-lactose-saccharose-agar (BPLS-agar) that is recommended by ISO 6579. The cultivation of about 5000 samples during the long-term monitoring (various tissues of died chicken, faeces, feedstuffs, water, environmental samples of the hen houses, the hatcheries, the slaughter-houses, and the feed mill as well as poultry meat) showed that the TBG-broth (sensitivity 99,1%) was considerably more sensitive than the RV-broth (sensitivity 74,9%). As for the selective agars, the salmonella detection rate was higher on the Rambach-agar than on the XLD-agar. The combination of the TBG-broth with the Rambach-agar showed a detection rate that was approximately as high as that of using two selective enrichment broths and two selective agars. Thus, the use of only TBG-broth and only Rambach-agar can be recommended for the monitoring of poultry meat production chains with diverse materials to be examined for salmonella.

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