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Publication type: Journal Article
Document type: Full Paper

Year: 2017

Author(s): Sassu, EL; Frömbling, J; Duvigneau, JC; Miller, I; Müllebner, A; Gutiérrez, AM; Grunert, T; Patzl, M; Saalmüller, A; von Altrock, A; Menzel, A; Ganter, M; Spergser, J; Hewicker-Trautwein, M; Verspohl, J; Ehling-Schulz, M; Hennig-Pauka, I

Title: Host-pathogen interplay at primary infection sites in pigs challenged with Actinobacillus pleuropneumoniae.

Source: BMC Vet Res. 2017; 13(1):64

Authors Vetmeduni Vienna:

Duvigneau Catharina
Ehling-Schulz Monika
Frömbling Janna
Grunert Tom
Hennig-Pauka Isabel
Miller Ingrid
Müllebner Andrea
Patzl Martina
Saalmüller Armin
Sassu Elena Lucia
Spergser Joachim

Vetmed Research Units
Institute of Microbiology, Functional Microbiology Division
Institute for Medical Biochemistry
Institute of Immunology
University Clinic for Swine

Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia and causes significant losses in the pig industry worldwide. Early host immune response is crucial for further progression of the disease. A. pleuropneumoniae is either rapidly eliminated by the immune system or switches to a long-term persistent form. To gain insight into the host-pathogen interaction during the early stages of infection, pigs were inoculated intratracheally with A. pleuropneumoniae serotype 2 and humanely euthanized eight hours after infection. Gene expression studies of inflammatory cytokines and the acute phase proteins haptoglobin, serum amyloid A and C-reactive protein were carried out by RT-qPCR from the lung, liver, tonsils and salivary gland. In addition, the concentration of cytokines and acute phase proteins were measured by quantitative immunoassays in bronchoalveolar lavage fluid, serum and saliva. In parallel to the analyses of host response, the impact of the host on the bacterial pathogen was assessed on a metabolic level. For the latter, Fourier-Transform Infrared (FTIR-) spectroscopy was employed.

Keywords Pubmed: Actinobacillus Infections/immunology
Actinobacillus Infections/metabolism
Actinobacillus Infections/microbiology
Actinobacillus Infections/veterinary*
Actinobacillus pleuropneumoniae*/immunology
Actinobacillus pleuropneumoniae*/isolation & purification
Actinobacillus pleuropneumoniae*/metabolism
Swine Diseases/immunology
Swine Diseases/metabolism
Swine Diseases/microbiology*

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