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Type of publication: Journal Article
Type of document: Data Article

Year: 2017

Authors: Nöbauer, K; Hummel, K; Mayrhofer, C; Ahrens, M; Setyabudi, FMC; Schmidt-Heydt, M; Eisenacher, M; Razzazi-Fazeli, E

Title: Comprehensive proteomic analysis of Penicillium verrucosum.

Source: Proteomics. 2017; 17(9):

Authors Vetmeduni Vienna:

Hummel Karin
Itze-Mayrhofer Corina
Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology

Mass spectrometric identification of proteins in species lacking validated sequence information is a major problem in veterinary science. In the present study, we used ochratoxin A producing Penicillium verrucosum to identify and quantitatively analyze proteins of an organism with yet no protein information available. The work presented here aimed to provide a comprehensive protein identification of P. verrucosum using shotgun proteomics. We were able to identify 3631 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally, a sequential window acquisition of all theoretical fragment-ion spectra analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning (day 3) and at the end of the log phase (day 12).© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords Pubmed: Databases, Protein
Fungal Proteinsmetabolism
Penicilliumclassificationgrowth & developmentmetabolism
Sequence Analysis, Proteinmethods

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